TSSC3 (PHLDA2) Goat Polyclonal Antibody

CAT#: AP31981PU-N

TSSC3 (PHLDA2) goat polyclonal antibody, Aff - Purified

Product Images

PHLDA2 antibody staining of paraffin embedded Human Placenta at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution:

  Peptide ELISA: 1/32000 (Detection limit).
  Western blot: Preliminary experiments gave no signal but low background in Jurkat and human placenta extracts at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
  Immunohistochemistry on Paraffin Sections: 3-6 μg/ml. In paraffin embedded Placenta shows staining of the syncytiotrophoblasts.

• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the C-terminus of Human PHLDA2 (NP_003302.1).
• Specificity: This antibody recognizes PHLDA2 at C-term.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: pleckstrin homology like domain family A member 2
• Database Link:
  Entrez Gene 7262 Human
  UniProt ID Q53GA4
• Background: TSSC3 in encoded in the imprinted gene domain of 11p15.5 which is considered to be an important tumor suppressor gene region. Alterations in this region may be associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Studies of the mouse gene, however, which is also located in an imprinted gene domain, have shown that the product of this gene regulates placental growth.
• Synonyms: BWR1C, HLDA2, IPL