SLC10A2 (C-term) Goat Polyclonal Antibody

CAT#: AP31971PU-N

SLC10A2 (C-term) goat polyclonal antibody, Aff - Purified

Product Images

SLC10A2 antibody staining of paraffin embedded Human Small Intestine at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution:

  Peptide ELISA: 1/16000 (Detection limit).
  Western blot: Preliminary experiments gave an approx. 60kDa band in Human Kidney lysates after 0.1 µg/ml antibody staining.
  Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 37.7kDa according to NP_000443.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide.
  Immunohistochemistry on Paraffin Sections: 3-6 μg/ml. In paraffin embedded Human Small Intestine shows staining of cells in the laminia propria between the intestinal vili.

• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide with sequence from the C-Terminus of Human SLC10A2 (NP_000443.1).
• Specificity: This antibody detects SLC10A2 at C-term.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: solute carrier family 10 member 2
• Database Link:
  Entrez Gene 6555 Human
  UniProt ID Q12908
• Background: This gene encodes a sodium/bile acid cotransporter. This transporter is the primary mechanism for uptake of intestinal bile acids by apical cells in the distal ileum. Bile acids are the catabolic product of cholesterol metabolism, so this protein is also critical for cholesterol homeostasis. Mutations in this gene cause primary bile acid malabsorption (PBAM); muatations in this gene may also be associated with other diseases of the liver and intestines, such as familial hypertriglyceridemia (FHTG). [provided by RefSeq]
• Synonyms: ASBT, ISBT, NTCP2, IBAT