Alb Rabbit Polyclonal Antibody

CAT#: AP31514SU-N

Alb rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Can be used as precipitating antiserum to identify or measure rat albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.
  Directions for use: In immunoelectrophoresis use 2 μl serum, plasma or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion and electroimmunodiffusion use 0.5 to 1.0 percent antiserum in
  the agar gel.
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly purified albumin isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The defined antibody specificity is directed to albumin as tested against rat sera. In immunoelectrophoresis
  and double radial immunodiffusion (Ouchterlony), using various antiserum
  concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin
  line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum
  and the purified albumin.
  Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since
  homologous proteins of different species frequently share antigenic determinants. The degree of
  cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the
  assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial
  immunodiffusion against several species sera with the following results:
  Cross-reactivity
  ++ guinea pig
  ± horse, human, swine, monkey
  + mouse
  - rabbit, sheep, goat, canine, chicken, bovine
• Formulation: No preservative added. - No foreign proteins added.
  State: Serum
  State: Delipidated, heat inactivated, lyophilized, stable whole antiserum
• Reconstitution Method: Restore with 1 ml sterile distilled water
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one week
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: albumin
• Database Link:
  Entrez Gene 24186 Rat
  UniProt ID P02770
• Synonyms: ALB, BSA, HSA, Serum Albumin
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.