GFAP (C-term) Rabbit Polyclonal Antibody

CAT#: AP23244PU-N

GFAP (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Polyclonal Anti-Glial fibrillary acidic protein GFAP Antibody

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 0.1-0.5 µg/ml.
  Immunohistochemistry on Paraffin Sections: 0.5-1 µg/ml.
  Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a sequence at the C-terminal (417-432aa) of Human GFAP.
• Specificity: This antibody detects GFAP at C-term. No cross reactivity with other proteins.
• Formulation: 0.2 mg Na2HPO4, 0.9 mg NaCl
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
  Stabilizer: 5 mg BSA
  Preservative: 0.05 mg Thimerosal, 0.05 mg Sodium Azide
• Reconstitution Method: 0.2 ml of distilled water will yield a concentration of 0.5 mg/ml.
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: glial fibrillary acidic protein
• Database Link:
  Entrez Gene 14580 MouseEntrez Gene 24387 RatEntrez Gene 2670 Human
  UniProt ID P14136
• Background: GGlial fibrillary acidic protein (GFAP) is an intermediate-filament (IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. GFAP has been located in rat kidney glomeruli and peritubular fibroblasts, leydig cells of testis, skin keratinocytes, osteocytes of bones, chondrocytes of epiglottis, bronchus, and stellate-shaped cells of the pancreas and liver. Its expression is essential for normal white matter architecture and blood-brain barrier integrity, and its absence leads to late-onset CNS dysmyelination. GFAP has also been shown to play a role in mitosis by adjusting the filament network present in the cell. During mitosis, there is an increase in the amount of phosphorylated GFAP, and a movement of this modified protein to the cleavage furrow.
• Synonyms: Glial Fibrillary Acidic Protein

Reference Data

• Protein Families: ES Cell Differentiation/IPS