ETFB (152-165) Goat Polyclonal Antibody

CAT#: AP21283PU-N

ETFB (152-165) goat polyclonal antibody, Aff - Purified

Product Images

ETFB antibody staining of Human Liver lysate at 0.1 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  ETFB antibody staining paraffin embedded Human Liver at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/8000 (Detetion Limit).
  Western blot: 1-3 µg/ml. This antibody detects a band of ~27kDa in Human, Mouse and Rat Liver lysates.
  Immunohistochemistry on Paraffin Sections: 3-5 µg/ml. In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes.
• Reactivities: Bovine, Canine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region (aa 152-165) of the protein sequence according to NP_001976.1 and NP_001014763.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001976.1 and NP_001014763.1).
• Formulation: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: electron transfer flavoprotein beta subunit
• Database Link:
  Entrez Gene 110826 MouseEntrez Gene 292845 RatEntrez Gene 2109 Human
  UniProt ID P38117
• Background: ETFB is a is an electron-transfer-flavoprotein, (beta polypeptide), and a subcomponent of Electron transfer flavoprotein (ETF). ETF exists in the mitochondrial matrix as a heterodimer of 30-kD alpha subunits (ETFA) and 28-kD beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer.
  This electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases and transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
• Synonyms: Beta-ETF
• Note: Calculated Molecular Weight: 27.8kDa (NP_001976.1).

Reference Data

• Protein Families: Druggable Genome