PTP alpha (PTPRA) (N-term) Rabbit Polyclonal Antibody

CAT#: AP15220PU-N

PTP alpha (PTPRA) (N-term) rabbit polyclonal antibody, Purified

Product Images

The anti-PTPalpha N-term Pab is used in Western blot to detect PTPalpha in mouse brain tissue lysate.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Peptide ELISA: 1/1,000.
  Western blotting: 1/1,000.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 89-120 amino acids from the N-terminal region of human PTP alpha.
• Specificity: This antibody reacts to PTPalpha.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein tyrosine phosphatase, receptor type A
• Database Link:
  Entrez Gene 5786 Human
  UniProt ID P18433
• Background: Phosphorylation of receptors by protein kinases is a process that can be reversed by a group of enzymes called protein phosphatases. Coordinated control of kinases and phosphatases provides the cell with the capacity to rapidly switch between phosphorylated and dephosphorylated protein states in dynamic response to environmental stimuli. Activation of critical enzymes by kinase phosphorylation alone is not enough to provide adequate regulation ? it is the combination with phosphatase dephosphorylation that effectively creates on/off switches to control cellular events. Errors in control, either through kinases or their counterpart phosphatases, can lead to unchecked cell growth attributable to human cancers and developmental disorders. Potential mechanisms to control dephosphorylation include changes in the expression of protein phosphatases, their subcellular localization, phosphorylation of phosphatase catalytic and regulatory subunits and regulation by endogenous phosphatase inhibitors. Most protein phosphatases are not stringently specific for their substrates. Consequently, changes in phosphatase activity may have a broad impact on dephosphorylation and turnover of phosphoproteins that are substrates for different kinases. This may be an important point of control to connect cellular circuitry of interrelated signaling pathways, and to synchronize physiological responses.
• Synonyms: PTPA, PTP alpha, R-PTP-alpha, PTPRL2, Protein-tyrosine phosphatase alpha