Aurora A (AURKA) (N-term) Rabbit Polyclonal Antibody

CAT#: AP14756PU-N

Aurora A (AURKA) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of AIK (arrow) using Aurora kinase A antibody in A375 cell lysate. AIK (arrow) was detected using purified Pab. Secondary HRP-anti-Rabbit was used for signal visualization with chemiluminescence.

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  Formalin-Fixed, Paraffin-Embedded Human cancer (Left) and Human colon carcinoma (Right) tissues reacted with Aurora Kinase A antibody using peroxidase-conjugated secondary antibody followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/1,000.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 90-120 amino acids from the N-terminal region of human AIK.
• Specificity: This antibody recognizes Aurora kinase A.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: aurora kinase A
• Database Link:
  Entrez Gene 6790 Human
  UniProt ID O14965
• Background: Aurora A (AIK) plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.
• Synonyms: AURKA, AIK1, ARK1, AURA, BTAK, STK15, STK6, Aurora/IPL1-related kinase 1, AURORA2