ART3 (C-term) Rabbit Polyclonal Antibody

CAT#: AP12147PU-N

ART3 (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of ART3 Antibody (C-term) in HL60 cell line lysates (25ug/lane). ART3 (arrow) was detected using the purified Pab (4 ug/ml).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1,000.
  western Blot: 1/100-1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ART3.
• Specificity: This antibody is specific to ART3/TMART (C-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ADP-ribosyltransferase 3
• Database Link:
  Entrez Gene 419 Human
  UniProt ID Q13508
• Background: Mono-ADP-ribosylation involves the transfer of the ADP-ribose moiety from NAD+ to a specific amino acid in the target protein. The rodent mono-ADP-ribosyltransferase RT6 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein specifically expressed at the cell surface of rat and mouse T lymphocytes. The predicted 367-amino acid human ART3 protein has an estimated molecular mass of 41.5 kD and contains a hydrophobic peptide signal at its N terminus, 3 consensus motifs specific to enzymes catalyzing ADP-ribose transfer, a hydrophobic C-terminal sequence characteristic of a GPI-anchored protein, a novel motif repeated 3 times at its C terminus, and 1 potential glycosylation site.1 The ART3 and rodent RT6 proteins share 35% amino acid identity.
• Synonyms: FLJ26404; mono-ADP-ribosyltransferase; TMART
• Note: Predicted Molecular weight: 43923 Da