CNOT4 (C-term) Rabbit Polyclonal Antibody

CAT#: AP11452PU-N

CNOT4 (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of CNOT4 Antibody (C-term) in CEM cell line lysates (35 ug/lane). CNOT4 (arrow) was detected using the purified Pab (1:60 dilution).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA 1:1,000.
  Western blot 1:50 - 1:100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected from the C-terminal region of human CNOT4A
• Specificity: This antibody detects CNOT4 T C-term.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CCR4-NOT transcription complex subunit 4
• Database Link:
  Entrez Gene 4850 Human
  UniProt ID O95628
• Background: CNOT4 has intrinsic E3 ubiquitin ligase activity. Binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective.The CCR4-NOT complex functions as a global regulator of RNA polymerase II transcription.
• Synonyms: NOT4Hp, NOT4
• Note: Molecular weight: 63510 Da