Chordin (CHRD) (C-term) Rabbit Polyclonal Antibody

CAT#: AP11375PU-N

Chordin (CHRD) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of anti-CHRD (C-term) in HepG2 cell line lysates (35 ug/lane). CHRD (arrow) was detected using the purified Pab (1:60 dilution).

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  Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CHRD antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA 1:1,000.
  Western blot 1:50 - 1:100.
  Immunohistochemistry 1:10 - 1:50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CHRD.
• Specificity: This antibody detects CHRD at C-term.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: chordin
• Database Link:
  Entrez Gene 8646 Human
  UniProt ID Q9H2X0
• Background: CHRD is a secreted protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. This protein may also have roles in organogenesis and during adulthood.
• Synonyms: CHRD
• Note: Molecular weight: 102032 Da