Cytokeratin 10 (KRT10) Guinea Pig Polyclonal Antibody

CAT#: AP09544SU-N

Cytokeratin 10 (KRT10) guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot.
  Immunofluorescence Microscopy on Frozen Sections.
  Immunohistochemistry on Paraffin Sections (After Microwave treatment).
  Working Dilution: 1/100-1/200 for Immunohistochemistry.
  Incubation Time: 1h at RT.
  Positive Control: Human suprabasal keratinocytes of epidermis; squamous cell carcinoma.
• Reactivities: Human, Mouse, Rat
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of Human Keratin K10 (formerly also designated Cytokeratin 10; C-GS VGE SSS KGP RY), coupled to KLH
• Specificity: MW 56,5000 (pI 5.3) intermediate filament polypeptide, keratin K10, detected by Immunohistochemistry in the suprabasal layers of Human epidermis (basal cells are negative).
  Useful for the recognition of keratinizing cells in squamous cell carcinoma of epidermis, lung, bladder, cervix, esophagus etc.
  Completely negative on non-stratified epithelia.
• Formulation: State: Serum
  State: Liquid Serum with 0.09% Sodium Azide and 0.5% BSA
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: keratin 10
• Database Link:
  Entrez Gene 16661 MouseEntrez Gene 450225 RatEntrez Gene 3858 Human
  UniProt ID P13645
• Background: Cytokeratin 10 is a heterotetramer of two type I and two type II keratins. Cytokeratin 10 is generally associated with keratin 1. It is seen in all suprabasal cell layers including stratum corneum. A number of alleles are known that mainly differ in the Gly-rich region (positions 490-560). Defects in cytokeratin 10 are a cause of epidermolytic hyperkeratosis (EHK), also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. Defects in cytokeratin 10 are also a cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens.
• Synonyms: Cytokeratin-10, CK10, CK-10, Keratin-10, KRT10, KPP, K10