Zbtb7b Rabbit Polyclonal Antibody

CAT#: AP09084SU-N

Zbtb7b rabbit polyclonal antibody, Serum

Product Images

Western blot using anti-cKrox antibody to detect endogenous cKrox in mouse thymocyte whole cell lysates. The left lane is a control with no primary antibody. The center lane is a reagent control. The right lane shows a band at 80 kDa corresponding to mouse cKrox protein. The membrane was probed with the primary antibody at a 1:500 dilution. Personal Communication, L. Wang and R. Bossulet, National Cancer Institute, Bethesda, MD.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1:2,500 - 1:10,000.
  Western blot: 1:500 - 1:3,000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to an internal sequence of mouse cKrox protein
• Specificity: This antibody is directed against mouse cKrox.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
  State: Serum
  State: Lyophilized delipidated and defibrinated antiserum
• Reconstitution Method: Reconstitute with 0.1 ml of deionized water.
• Conjugation: Unconjugated
• Storage: Store vial at 2-8° C prior to opening. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute
  only prior to immediate use.
• Stability: Shelf life: One year from despatch.
• Gene Name: zinc finger and BTB domain containing 7B
• Database Link:
  Entrez Gene 22724 Mouse
  UniProt ID Q64321
• Background: cKrox (also known as Zfp67 or ThPOK) functions as a transcription regulator that acts as a key modulator of lineage commitment for immature T-cell precursors. cKrox is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. cKrox is a known transcriptional repressor of the collagen COL1A1 and COL1A2 genes and may also function as a repressor of fibronectin and possibly other extracellular matrix genes. cKrox is located within the nucleus and is expressed in multiple tissues and organs including skin, thymus and peripheral T-cells.
• Synonyms: ZFP67, Zfp-67, ZBTB15, ZBT-15, Thpok, Th-POK, cKrox, c-Krox