Bcl2 Binding component 3 (BBC3) (C-term) Rabbit Polyclonal Antibody

CAT#: AP07278PU-N

Bcl2 Binding component 3 (BBC3) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Formalin-Fixed Paraffin-Embedded Human Breast stained with PUMA Antibody at 10 ug/ml after heat-induced antigen retrieval.

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  Formalin-Fixed Paraffin-Embedded Small intestine stained with PUMA Antibody at 10 ug/ml after heat-induced antigen retrieval.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunocytochemistry.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot: 1 - 2 µg/ml.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the C-terminal region of Human PUMA
• Specificity: This antibody recognizes a synthetic peptide corresponding to 14 amino acids near the carboxy terminus of PUMA-alpha. This sequence is identical between alpha and beta forms of the PUMA proteins.
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: BCL2 binding component 3
• Database Link:
  Entrez Gene 27113 Human
  UniProt ID Q96PG8
• Background: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
• Synonyms: BBC3, JFY-1