SPT3 (SUPT3H) Rabbit Polyclonal Antibody

CAT#: AP06645PU-N

SPT3 (SUPT3H) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of SPT3 antibody in extracts from HUVEC cells.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Westen blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 181-230 of Human SPT3.
• Specificity: This antibody detects endogenous levels of SPT3 protein.
  (region surrounding Glu203)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 50 kDa
• Gene Name: SPT3 homolog, SAGA and STAGA complex component
• Database Link:
  Entrez Gene 8464 Human
  UniProt ID O75486
• Background: The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from δ sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter allowing TBP to bind to SAGA-dependent promoters. Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3 and TRP3 genes, Spt3 inhibits binding of TBP, resulting in reduced transcription. This repressive effect of Spt3 can be overcome by another member of the SAGA complex, GCN5, which promotes the formation of a TBP/TFIIA complex by histone acetylation.
• Synonyms: SPT3-like protein, SPT3L

Reference Data

• Protein Families: Stem cell - Pluripotency, Transcription Factors