Notum Rabbit Polyclonal Antibody

CAT#: AP05257PU-N

Notum rabbit polyclonal antibody, Purified

Product Images

Western blot of HepG2 cell lysate (11 ug/lane) using (10 ug/ml). Blots were developed with anti Rabbit HRP 1:100k and using Pierce Super Signal West Femto.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 5-10 µg/ml.
  Immunohistochemistry.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant full-length notum protein from Mouse
• Specificity: This antibody detects Notum.
• Formulation: Phosphate buffered saline with 0.08% Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the product (in aliquots) at -20°C.
  Can be shipped at 2-8°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: notum pectinacetylesterase homolog (Drosophila)
• Database Link:
  Entrez Gene 77583 Mouse
  UniProt ID Q8R116
• Background: Genetic studies in Drosophila have revealed a novel secreted antagonist for Wingless (Wg) signaling, referred to as Notum/Wingful (Wf). Wf/Notum encodes a protein of 430 amino acid residues, which is related to plant pectin acetylesterases of the alpha/beta-hydrolase superfamily. The main target of Notum action appears to be cell surface proteoglycans Dally and/or Dally-like (Dlp). Notum governs the extracellular Wg morphogen distribution via Dally and Dlp. Also, like some vertebrate Wnt antagonists, notum expression is activated by Wg signaling, revealing the existence of a feedback control mechanism in which Wg modifies its own gradient distribution. In vertebrate, proteoglycans related to Dally and Dlp likely play important roles in Wnt signaling which encodes a glypican that is required for Wnt-11 signaling. A single ortholog of Notum exists in the mouse and the human genome, implying that a similar regulatory mechanism may exist in vertebrates.
• Synonyms: NOTUM