beta Catenin (CTNNB1) Rabbit Polyclonal Antibody

CAT#: AP02722PU-S

beta Catenin (CTNNB1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from HT29 and SKOV3 cells using beta-Catenin antibody

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using β-Catenin antibody.

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  Immunofluorescence staining of methanol-fixed HeLa cells using beta-Catenin antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/1000; Incubate membrane with diluted antibody in 5% nonfat milk, 1X TBS, 0,1% Tween-20 at 4°C with gentle shaking, overnight.
  Immunohistochemistry on paraffin sections: 1/50 - 1/100.
  Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around aa. 35~39 (I-H-S-G-A) derived from Human b-Catenin.
• Specificity: beta-Catenin antibody detects endogenous levels of total beta-Catenin protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: catenin beta 1
• Database Link:
  Entrez Gene 1499 Human
  UniProt ID P35222
• Background: Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
  Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
• Synonyms: CTNNB1, CTNNB, Beta-catenin