Nid1 Rabbit Polyclonal Antibody

CAT#: AP02274SU-N

Nid1 rabbit polyclonal antibody, Serum

Product Images

Western Blot analysis of Nidogen 1 antibody Cat.-No AP02274SU specificity. Protein extracts of Heart lung and Skeletal muscle from normal (+/+) and Nidogen 2-mutant (-/-) mice were separated by SDS-PAGE and immunoblotted with AP02274SU (1/1000). This antibody detects several Nidogen 1 specific bands (arrow heads) in normal and Nidogen 2 deficient mice Heart (H), Lung (L), Muscle (M). Schymeinsky J et al. (2002) Mol Cell Biol 22 (19): 6820-6830.

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  Immunofluorescence staining in Cryosection of Mouse cartilage and surounding tissue using Nidogen 1 antibody Cat.-No AP02274SU. Schymeinsky J et al. (2002) Mol Cell Biol 22 (19): 6820-6830.

Specifications

Product Data

• Applications: ELISA, IF, IHC, R, WB
• Recommended Dilution: RIA (Ref.1).
  ELISA (Ref.2).
  Western blot: 1/1000 (Ref.1,3,4).
  Immunofluorescence.
  Immunohistochemistry on Frozen Sections (Ref.2,3).
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Mouse Nidogen 1
• Specificity: This antibody detects Mouse Nidogen 1. Other species not tested.
• Formulation: State: Serum
  State: Lyophilized Serum
• Reconstitution Method: Restore in aqua bidest to initial volume.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: nidogen 1
• Database Link:
  Entrez Gene 18073 Mouse
  UniProt ID P10493
• Background: Nidogen 1 / Entactin is a sulfated glycoprotein which is widely distributed in basement membranes and that is tightly associated with laminin. Also binds to collagen IV. It probably has a role in cell extracellular matrix interactions.
• Synonyms: NID1, NID, Entactin
• Note: LocusID 18073