CCN3 (NOV) Rabbit Polyclonal Antibody

CAT#: AP01154BT-N

CCN3 (NOV) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: Direct: To detect hNOV (using 100 μl/well antibody solution) a concentration of
  0.25 - 1.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNOV.
  Sandwich: To detect hNOV (using 100 μl/well antibody solution) a concentration of
  0.25 - 1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human NOV as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNOV.
  Western blot: To detect hNOV this antibody can be used at a concentration of
  0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNOV is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98 %) recombinant human NOV
• Specificity: This antibody detects NOV.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Sterile filtered lyophilized Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/ml.
• Purification: Affinity chromatography
• Conjugation: Biotin
• Storage: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at
  2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: nephroblastoma overexpressed
• Database Link:
  Entrez Gene 4856 Human
  UniProt ID P48745
• Background: CCN3 is a cysteine-rich protein that is overexpressed in avian nephroblastomas. It is a member of the CCN family of proteins that includes CTGF. These proteins are encoded by a group of genes known as immediate-early genes, so named because they are expressed after induction by growth factors or certain oncogenes. The proteins share several common structural motifs: a consensus sequence present in IGF (insulin-like growth factor)-binding proteins (the IGFBP motif), an oligomeric complex-forming domain first identified in von Willebrand factor, a binding domain to soluble and matrix molecules and a dimerization (CT) domain. All CCN family members are thought to be involved in the control of cell proliferation.
• Synonyms: CCN3, IGFBP9, NOVH, NovH