IL31 Rabbit Polyclonal Antibody

CAT#: AP01149BT-N

IL31 rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: Direct: To detect hIL-31 (using 100 μl/well antibody solution) a concentration of
  0.25 - 1.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-31.
  Sandwich: To detect hIL-31 (using 100 μl/well antibody solution) a concentration of
  0.25 - 1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human IL-31 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-31.
  Western blot: To detect hIL-31 this antibody can be used at a concentration of
  0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-31 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (> 98 %) recombinant human IL-31
• Specificity: This antibody detects Interleukin-31.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Sterile filtered lyophilized Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/ml.
• Purification: Affinity chromatography
• Conjugation: Biotin
• Storage: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at
  2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: interleukin 31
• Database Link:
  Entrez Gene 386653 Human
  UniProt ID Q6EBC2
• Background: Interleukin-31 (IL-31) is a recently discovered T-cell cytokine closely related to IL-6 type cytokines and is preferentially produced by T helper type 2 cells. IL-31 activity is mediated through the ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor. In response to IL-31 binding, these proteins activate the JAK/STAT and the AKT signaling pathways. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes but are expressed constitutively in epithelial cells. IL-31, when overexpressed in transgenic mice, results in the development of pruritis, alopecia and skin lesions, and in humans may result in atopic dermatitis, suggesting that IL-31 may represent a novel target for antipruritic drug development.
• Synonyms: IL-31