DEP1 (PTPRJ) (1-444) Mouse Monoclonal Antibody [Clone ID: MEM-CD148/05]

CAT#: AM26703PU-N

DEP1 (PTPRJ) (1-444) mouse monoclonal antibody, clone MEM-CD148/05, Aff - Purified

Specifications

Product Data

• Clone Name: MEM-CD148/05
• Applications: FC
• Recommended Dilution: Flow Cytometry.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant CD148 (amino acids 1-444)
• Specificity: The antibody recognizes CD148, a highly glycosylated up to 250 kDa receptor-like protein tyrosin phosphatase expressed mainly in lymphocytes, myeloid cells and epithelial cells.
• Formulation: PBS, approx. pH 7.4
  State: Aff - Purified
  State: Liquid purified fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: protein tyrosine phosphatase, receptor type J
• Database Link:
  Entrez Gene 5795 Human
  UniProt ID Q12913
• Background: CD148 (also known as HPTP-eta or DEP-1) is a transmembrane protein tyrosin phosphatase containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leucocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes.
• Synonyms: DEP1, DEP-1, HPTP eta, R-PTP-eta, Protein-tyrosine phosphatase eta, R-PTP-J