RUNX2 Mouse Monoclonal Antibody [Clone ID: 1D2]

CAT#: AM20584PU-N

RUNX2 mouse monoclonal antibody, clone 1D2, Purified

Product Images

Detection limit for recombinant GST tagged RUNX2 is approximately 0.3 ng/ml as a capture antibody.

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  Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence of monoclonal antibody to RUNX2 antibody on HeLa cell at 10 ug/ml.

Specifications

Product Data

• Clone Name: 1D2
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunofluorescence: 10 µg/ml.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Partial Recombinant RUNX2 (NP_004339, 251 a.a. ~ 351 a.a) protein with GST tag.
• Specificity: Recognizes Runt-related Transcription Factor 2 (RUNX2).
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein A Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: runt related transcription factor 2
• Database Link:
  Entrez Gene 860 Human
  UniProt ID Q13950
• Background: RUNX family members are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX2 is essential for skeletal mineralization in that it stimulates osteoblast differentiation of mesenchymal stem cells, promotes chondrocyte hypertrophy and contributes to endothelial cell migration and vascular invasion of developing bones. Regulating RUNX2 expression may be a useful therapeutic tool for promoting bone formation. Mutations in the C-terminus of RUNX2 are associated with cleidocranial dysplasia syndrome, an autosomal-dominant skeletal dysplasia syndrome that is characterized by widely patent calvarial sutures, clavicular hypoplasia, supernumerary teeth, and short stature.
• Synonyms: Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, AML3, AML-3, CBFA1, OSF2, OSF-2, PEBP2A, PEA2-alpha A, PEBP2-alpha A

Reference Data

• Protein Families: Druggable Genome, Transcription Factors