Beclin 1 (BECN1) Mouse Monoclonal Antibody [Clone ID: 12B4]

CAT#: AM20210PU-N

Beclin 1 (BECN1) mouse monoclonal antibody, clone 12B4, Purified

Product Images

Detection of endogenous Beclin: Whole cell lysates of untreated tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with Beclin antibody (Clone 12B4) at 0.5 ug/ ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK 293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT Lane 8: Neuro2A

Specifications

Product Data

• Clone Name: 12B4
• Applications: WB
• Recommended Dilution: Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Included Potitive Control: Cell lysate from untreated Neuro 2A (See Protocols for more details).
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to hemocyanin derived from Beclin sequence.
• Specificity: This Monoclonal antibody recognizes Beclin at 52 kDa in Western-blot application.
• Formulation: PBS containing 0.09% Sodium Azide, PEG, Sucrose and 50% Glycerol.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.
• Conjugation: Unconjugated
• Storage: Upon receipt, store (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 52 kDa
• Gene Name: beclin 1
• Database Link:
  Entrez Gene 56208 MouseEntrez Gene 114558 RatEntrez Gene 8678 Human
  UniProt ID Q14457
• Background: Beclin 1 is a ubiquitous coiled-coil protein that plays a central role in autophagy. It interacts with several cofactors (Ambra1, Bif1 , UVRAG) to activate the lipid kinase Vps34, thereby inducing autophagy. In normal conditions, the BH3 domain of Beclin 1 is bound to, and inhibited by, Bcl2 or Bcl-X(L). This interaction can be disrupted by BH3-only proteins to induce autophagy. Nutrient starvation, a potent physiological inducer of autophagy, can stimulate the dissociation of Beclin 1 from its inhibitors, either by activating BH3-only proteins, such as Bad, or by post-translational modifications of Bcl2.
  Beclin 1 may play a role in anti-viral host defence, and protects against infection by a neurovirulent strain of Sindbis virus. Beclin 1 is also a haploinsufficient tumour suppressor.
  Genetic studies revealed that Beclin-1 has structural similarity to the yeast autophagy gene, atg6. Beclin-1 is part of a complex with class III PI3 kinase, Vsp34, that is responsible for autophagosome formation.
• Synonyms: BECN1, GT197
• Note: Protocol: Positive Control: Cell lysate from untreated Neuro2A cells, brain endothelioma (Mouse)
  
  Format: Lyophilized cell lysate from serum starved Neuro 2A cells.
  
  Reconstitution: Restore by addition of 200 µl H₂O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
  Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
  Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.
  
  Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Regulation of autophagy