S100A12 Mouse Monoclonal Antibody [Clone ID: 19F5]

CAT#: AM10219PU-N

S100A12 mouse monoclonal antibody, clone 19F5, Purified

Specifications

Product Data

• Clone Name: 19F5
• Applications: ELISA, IHC, WB
• Recommended Dilution:

  ELISA: 1/2000-1/10000.
  Western Blot: 1/200-1/1000.
  Immunohistochemistry: 1/100-1/500.

• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant S100A12 protein
• Specificity: Reacts with 10 kDa Human S100A12 protein.
  No cross reaction with the other S100 protein family.
• Formulation: PBS
  State: Purified
  State: Lyophilized purified powder
  Preservative: None
• Reconstitution Method: Restore in distilled water.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: S100 calcium binding protein A12
• Database Link:
  Entrez Gene 6283 Human
  UniProt ID P80511
• Background: S100 family proteins are acidic low molecular weight proteins containing 2 EF-hand calcium binding domains. They are characterised by their cell-type specific expression patterns. S100A12 is a member of the calgranulin sub-family. Calgranulins are S100 proteins that are expressed by neutrophils and also found in infiltrating monocytes and granulocytes during chronic inflammation. S100A12 is expressed in neutrophils and monocytes. It is highly abundant in resting neutrophils, comprising 5% of total cytostolic protein. S100A12 is a ligand for RAGE. Interaction with RAGE on mononuclear phagocytes, lymphocytes and endothelium triggers cellular activation. Nuclear factor kappa B, a key transcription factor for inflammatory events, is activated by S100A12/RAGE. S100A12 has also been shown to have anti-microbial properties.
• Synonyms: S100-A12, Calgranulin-C, CAGC, CGRP, CAAF1, EN-RAGE