Cytokeratin 5 (KRT5) Mouse Monoclonal Antibody [Clone ID: 2C2]

CAT#: AM06747PU-N

Cytokeratin 5 (KRT5) mouse monoclonal antibody, clone 2C2, Purified

Product Images

Left: Western blot analysis using Cytokeratin 5 antibody against Human CK5 (AA: 158-272) recombinant protein (Expected MW is 33.3 kDa). Right: Western blot analysis using Cytokeratin 5 antibody against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded breast cancer (left) and stomach (right) tissues using Cytokeratin 5 antibody with DAB staining.

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  Left: Immunofluorescence analysis of HeLa cells. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Right: Immunofluorescence analysis of HeLa cells using Cytokeratin 5 antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Flow Cytometric analysis of HeLa cells using Cytokeratin 5 antibody (green) and negative control (red).

Specifications

Product Data

• Clone Name: 2C2
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human CK5 expressed in E. Coli.
• Specificity: Recognizes Cytokeratin 5
• Formulation: PBS
  State: Purified
  State: Liquid purified antibody
  Stabilizer: 0.5% protein stabilizer
  Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 62.3 kDa
• Gene Name: keratin 5
• Database Link:
  Entrez Gene 3852 Human
  UniProt ID P13647
• Background: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
• Synonyms: KRT5, Cytokeratin-5, Keratin-5, Keratin 5, CK5, K5