RAB25 Mouse Monoclonal Antibody [Clone ID: 3F12]

CAT#: AM06503SU-N

RAB25 mouse monoclonal antibody, clone 3F12, Ascites

Product Images

Western blot analysis using Rab25 antibody Cat.-No AM06503SU-N against MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded esophagus tissues (left) and human lung cancer (right) using Rab25 antibody Cat.-No AM06503SU-N with DAB staining.

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  Immunofluorescence analysis of A549 cells using RAB25 antibody Cat.-No AM06503SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Flow cytometric analysis of NIH/3T3 cells using Rab25 antibody Cat.-No AM06503SU-N (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 3F12
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Flow Cytometry: 1/200 - 1/400.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Rab25 expressed in E. Coli.
• Specificity: Recognizes RAB25
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 24 kDa
• Gene Name: RAB25, member RAS oncogene family
• Database Link:
  Entrez Gene 57111 Human
  UniProt ID P57735
• Background: Members of the Ras-related superfamily of GTP binding proteins, which includes Ras, Rho, Rab and ARF subfamilies, exhibit 30-50% similarity with Ras p21. Rab proteins play an important role for either in endocytosis or in biosynthetic protein transport. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% similar to Rab proteins, is associated with secretory vesicles. Rab proteins located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Rab25 was cloned from a gastric parietal cell cDNA library and is expressed in epithelial tissues such as the gastrointestinal mucosae, kidney, and lung, which encoded a protein of 28 kDa.
• Synonyms: Rab-25, CATX8, CATX-8