N Cadherin (CDH2) Mouse Monoclonal Antibody [Clone ID: 5D5]

CAT#: AM06440SU-N

N Cadherin (CDH2) mouse monoclonal antibody, clone 5D5, Ascites

Product Images

Western blot analysis using CDH2 antibody Cat.-No AM06440SU-N against A431 (1), NIH/3T3 (2), Hela (3), C6 (4) and LNCap (5) cell lysate.

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  Paraffin-Embedded Human Placenta tissues stained with CDH2 antibody Cat.-No AM06440SU-N

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  Immunohistochemical analysis of paraffin-embedded human lung cancer (A), colon cancer (B), ovarian cancer (C) and mammary cancer (D), using CDH2 antibody Cat.-No AM06440SU-N with DAB staining.

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  Immunofluorescence analysis of A431 cells using CDH2 antibody Cat.-No AM06440SU-N (green). Blue: DRAQ5 fluorescent DNA dye.

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  Flow cytometric analysis of PC-2 cells using CDH2 antibody Cat.-No AM06440SU-N (green) and negative control (purple).

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Specifications

Product Data

• Clone Name: 5D5
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Flow cytometry: 1/200 - 1/400.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human CDH2 expressed in E. Coli.
• Specificity: This antibody recognizes Cadherin-2 (CDH2).
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 99.8 kDa
• Gene Name: cadherin 2
• Database Link:
  Entrez Gene 1000 Human
  UniProt ID P19022
• Background: Cadherins comprise a family of Ca2⁺-dependent adhesion moleculaes that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy terminal intracellular domains.
• Synonyms: CDH2, CDHN, NCAD, Neural cadherin, CDw325, Cadherin N, N-Cadherin