Lysyl tRNA synthetase (KARS) Mouse Monoclonal Antibody [Clone ID: 8G12C1]

CAT#: AM06252SU-N

Lysyl tRNA synthetase (KARS) mouse monoclonal antibody, clone 8G12C1, Ascites

Product Images

Western blot analysis using KARS antibody Cat.-No AM06252SU-N against truncated Trx-KARS recombinant protein (Lane 1), truncated MBP-KARS (aa90-174) and full length KARS (aa1-188) transfected CHO-K1 cell lysate (Lane 3).

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  Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using KARS antibody Cat.-No AM06252SU-N

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  Immunohistochemical analysis of paraffin-embedded human cervical carcinoma, showing cytoplasmic localization with DAB staining using KARSantibody Cat.-No AM06252SU-N

Specifications

Product Data

• Clone Name: 8G12C1
• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western blot: 1/500 - 1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200-1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of KARS(aa90-174) expressed in E. Coli.
• Specificity: Recognizes Lysyl Trna Synthetase (KARS).
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: lysyl-tRNA synthetase
• Database Link:
  Entrez Gene 3735 Human
  UniProt ID Q15046
• Background: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.
• Synonyms: Lysyl-tRNA synthetase, KIAA0070, LysRS, Lysine-tRNA ligase