IKK beta (IKBKB) Mouse Monoclonal Antibody [Clone ID: 10A2]

CAT#: AM06154SU-N

IKK beta (IKBKB) mouse monoclonal antibody, clone 10A2, Ascites

Product Images

Western blot analysis using IKBKB antibody Cat.-No AM06154SU-N against truncated IKBKB recombinant protein (Lane 1) and K562 cell lysate (Lane 2).

Specifications

Product Data

• Clone Name: 10A2
• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of IKBKB expressed in E. Coli.
• Specificity: Recognizes IKBKB / IKKB
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 43 kDa
• Gene Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
• Database Link:
  Entrez Gene 3551 Human
  UniProt ID O14920
• Background: IKBKB (Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta, also called IKK2/IKKB), is a member of the IKK complex which is composed of IKK-alpha, IKK-beta, IKK-gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK-beta has been shown to activate NF-kB and phosphorylate IKB-alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK-beta is essential for activation of IKK by TNF and IL1. Once activated, IKK-beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK-beta activity can also be regulated by MEKK-1.
• Synonyms: I-kappa-B-kinase beta, IKK-beta, IKK-B, I-kappa-B kinase 2, IKK2, NFKBIKB