CD4 Mouse Monoclonal Antibody [Clone ID: MEM-16]

CAT#: AM03098PU-N

CD4 mouse monoclonal antibody, clone MEM-16, Purified

Specifications

Product Data

• Clone Name: MEM-16
• Applications: FC, IP
• Recommended Dilution: Flow Cytometry: 10 μg/ml
  Positive control: Peripheral blood lymphocytes
  Immunoprecipitation.
• Reactivities: Human
• Host: Mouse
• Isotype: IgM
• Clonality: Monoclonal
• Immunogen: HPB cell line (human peripheral blood leukemia T-cells).
• Specificity: The antibody MEM-16 recognizes an epitope EF loop of D1 domain of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (''helper'' T-cells) and also on monocytes, tissue macrophages and granulocytes. Negative Species: Porcine.
• Formulation: PBS, pH 7.4 with 15 mM sodium azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE).
• Concentration: lot specific
• Purification: Precipitation methods and size-exclusion chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD4 molecule
• Database Link:
  Entrez Gene 920 Human
  UniProt ID P01730
• Background: CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains.
  Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin
  Intracellular ligands: p56Lck
  CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
• Synonyms: T-cell surface antigen T4/Leu-3