PRKACA Mouse Monoclonal Antibody [Clone ID: 6D2.1]

CAT#: AM03041PU-N

PRKACA mouse monoclonal antibody, clone 6D2.1, Aff - Purified

Product Images

Dot Blot analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST. The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot. Lane 1: GST-Akt1 Lane 2: GST-Akt2 Lane 3: GST-Akt3 Lane 4: GST-PKAc alpha Lane 5: GST-PKAc beta Lane 6: GST-PKAc gamma Lane 7: GST-MEK 1 Lane 8: GST

Specifications

Product Data

• Clone Name: 6D2.1
• Applications: WB
• Recommended Dilution: Suitable for Western Blotting (0.5-1 μg/ml).
  Positive control: HeLa human cervix carcinoma cell line
  Note: To detect PKAc gamma, use a more concentrated lysate from a tissue expressing this subunit (testis).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha).
• Specificity: This antibody strongly reacts with human proteinkinase A catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa).
  The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.
• Formulation: PBS, pH 7.4 with 15 mM sodium azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
• Concentration: lot specific
• Purification: Protein A affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid freeze/thaw cycles.
• Stability: Shelf life: One year from despatch.
• Gene Name: protein kinase cAMP-activated catalytic subunit alpha
• Database Link:
  Entrez Gene 5566 Human
  UniProt ID P17612
• Background: Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.
• Synonyms: PKA C-alpha