Perforin (PRF1) Mouse Monoclonal Antibody [Clone ID: dG9]

CAT#: AM00176RP-N

Perforin (PRF1) mouse monoclonal antibody, clone dG9, PE

Product Images

Intracellular staining of perforin in human peripheral blood cells with anti-perforin (dG9) PE.

Specifications

Product Data

• Clone Name: dG9
• Applications: FC
• Recommended Dilution: Flow cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (1 ml) is sufficient for 100 tests.
• Reactivities: Bovine, Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified granules from human YT lymphoma cell line
• Specificity: This antibody recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
• Formulation: Phosphate buffered saline (PBS)
  Label: PE
  State: Liquid purified Ig fraction
  Stabilizer: 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA)
  Preservative: 15 mM sodium azide
  Label: Conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Conjugation: PE
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: perforin 1
• Database Link:
  Entrez Gene 5551 Human
  UniProt ID P14222
• Background: Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.
• Synonyms: Perforin-1, Pore forming protein, PFN, PFP, Cytolysin