Vimentin (VIM) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 11H6]

CAT#: AM00158PU-N

Vimentin (VIM) (incl. pos. control) mouse monoclonal antibody, clone 11H6, Purified

Product Images

Detection of endogenous Vimentin: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab Vim-11H6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A; lane 9: 3T3

Specifications

Product Data

• Clone Name: 11H6
• Applications: WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Included Positive Control: Cell lysate from Untreated HeLa cells (See Protocols for more details).
• Reactivities: Canine, Human, Mouse
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to hemocyanin.
  Epitope: N-terminus.
• Specificity: This antibody specifically recognizes Vimentin at 54 kDa in Western-blot applications.
• Formulation: 1ml 2 x PBS containing 0.09% Sodium Azide/PEG and Sucrose.
  State: Purified
  State: Lyophilized purified IgG fraction.
• Reconstitution Method: Restore with 1 ml H₂O (15 min, RT).
• Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 54 kDa
• Gene Name: vimentin
• Database Link:
  Entrez Gene 7431 Human
  UniProt ID P08670
• Background: Vimentin is a major subunit protein of intermediate filaments found in mesenchymal cells. Vimentin is expressed in many tumors/tumor cell lines after epithelial to mesenchymal transition.
• Synonyms: VIM
• Note: Protocol: Positive Control Provided:
  Cell lysate from Untreated HeLa cells.
  Lyophilized cell lysate from serum starved HeLa cells.
  
  Format: Lyophilized cell lysate from HeLa cells.
  
  Reconstitution: Restore by addition of 200 μl H20. After complete solubilization add 200 μl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Storage: Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  
  Application: The positive control cell lysate is recommended for immunoblot applications.
  20 μl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 μl / lane (mini gel) for HRPO/ECL detection of the target proteins.
  
  Please note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.