PKA Substrate pSer (activated) Mouse Monoclonal Antibody [Clone ID: 15B12]

CAT#: AM00105PU-N

PKA Substrate pSer (activated) mouse monoclonal antibody, clone 15B12, Purified

Specifications

Product Data

• Clone Name: 15B12
• Applications: ELISA
• Recommended Dilution: ELISA: 0.1-1.0 µg/ml.
  DO NOT USE MILK OR CASEIN FOR BLOCKING AND DILUTION! REPLACE BY BSA!
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjuagted to KLH. Epitope: R-R-V-pS-N
• Specificity: Monoclonal antibody raised against the sequence R-R-V-pS-N derived from Human VASP protein.
  The antibody is recommended for use in non-radioactive quantification of Phosphopeptides in kinase/Phosphatase assays.
• Formulation: 1 ml 2 x PBS containing 0.09% Sodium Azide/ PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 1 ml H₂O (15 min, RT)
• Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.