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Anti-tGFP magnetic beads, 1ml
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|Product Name||Anti-tGFP magnetic beads, 1ml|
|Clone Name||clone OTI2H8 (formerly 2H8)|
|Immunogen||Anti-tGFP monoclonal antibody was produced by immunizing mice with purified tGFP protein expressed in HEK293T cell. |
|Formulation||PBS (pH7.4) containing 50% Glycerol, 0.1% BSA, 0.02% NaN3 with final concentration 5mg/mL Beads|
|Validated Application||IP, Protein purification|
|Suggested Dilution||IP: see datasheet|
|Storage/Stability||Stable for 1 year at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing.|
Immunoprecipitation (IP) was performed with overexpression lysate expressed with tGFP expression vector (Cat# PS100010) or control lysate transfected with pCMV-Entry empty vector (Cat# PS100001). Briefly, 20uL anti-tGFP (2H8) magnetic beads (Cat# TA150039) were incubated with tGFP overexpression lysate or negative control lysate overnight at 4°C, respectively. After washing three times with RIPA buffer, 20uL 4x SDS sample buffer was applied to the lysate-beads mixture. The samples were boiled at 95°C for 10min. The IP sample supernatants were fractionated on SDS-PAGE gel, blotted onto nitrocellulose membrane. The IP efficiency was evaluated by using HRP-conjugated anti-tGFP antibody (Cat# TA150043). Lane 1: anti-tGFP (2H8) magnetic beads (20uL) with tGFP expression lysate; Lane 2: anti-tGFP magnetic beads (20uL) with negative control lysate.