Home Antibody Antitag Antibodies
Anti-HA tag rabbit polyclonal antibody
|Product Name||Anti-HA tag rabbit polyclonal antibody|
|Immunogen||Synthetic peptide contain a sequence corresponding to a region of HA Tag|
|Formulation||1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Validated Application||WB, IP, IF|
|Suggested Dilution||WB: 1:500-1:3000, ICC/IF: 1:100-1:500, IP:1:500|
|Storage/Stability||Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.|
Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing HA-tagged protein (~17 kDa) using HA-tag antibody. A: Non-transfected lysate. B: Transfected lysate.
Immunofluorescence analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using HA Tag antibody (TA150034) at 1:500 dilution.
HA-tagged protein was immunoprecipitated from transfected cells using 5 ug of anti-HA tag antibody (TA150034). The precipitated HA-tagged protein was detected by TA150034 diluted at 1:1000, and used anti-rabbit IgG as secondary reagent. 12% SDS PAGE, TA150034 diluated at 1:2000.