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Home Antibody Antitag Antibodies

Anti-HA Tag Antibody

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Specifications Citations Related Products Product Documents
SKU Description Datasheet Price Availability  
TA150034 Anti-HA tag rabbit polyclonal antibody, 100ul $248 In Stock Add to Shopping Cart

Specification

Product NameAnti-HA tag rabbit polyclonal antibody
Clone NamePolyclonal
IsoTypeIgG
Concentration1mg/ml
ImmunogenSynthetic peptide contain a sequence corresponding to a region of HA Tag
Host SpeciesRabbit
Formulation1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
ReactivityHA tag
Validated ApplicationWB, IP, IF
Suggested DilutionWB: 1:500-1:3000, ICC/IF: 1:100-1:500, IP:1:500
Predicted Size
Storage/StabilityKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
WB Image
Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing HA-tagged protein (~17 kDa) using HA-tag antibody. A: Non-transfected lysate. B: Transfected lysate.
IF Image
Immunofluorescence analysis of HA-recombinant protein transfected HeLa cell which fixed with paraformaldehyde, using HA Tag antibody (TA150034) at 1:500 dilution.
IP Image
HA-tagged protein was immunoprecipitated from transfected cells using 5 ug of anti-HA tag antibody (TA150034). The precipitated HA-tagged protein was detected by TA150034 diluted at 1:1000, and used anti-rabbit IgG as secondary reagent. 12% SDS PAGE, TA150034 diluated at 1:2000.

 

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