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Rabbit polyclonal eGFP antibody
|Product Name||Rabbit polyclonal eGFP antibody|
|Immunogen||Recombinant fragment contain a sequence corresponding to a region within amino acids 70 and 265 of GFP|
|Formulation||1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative|
|Validated Application||WB, IF, IP|
|Suggested Dilution||WB: 1:5000-1:10000, ICC/IF: 1:1000-1:5000, IP: 1:100-1:500|
|Storage/Stability||Stable for 1 year at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing.|
Western blot analysis of GFP expression in transfected 293T cell line by GFP polyclonal antibody (12% SDS-PAGE, TA150032 diluted at 1:10000). A: Non-transfected lysate; B: GFP transfected lysate.
Western blot analysis of 1 ug of whole cell lysate with anti-GFP antibody TA150032 diluted at 1:10000. A: Non-transfected 293T lysates; B: GFP transfected 293T lysates; C: RFP transfected 293T lysates.
Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( TA150032, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.
GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (TA150032, 1:15000).