|
Home Antibody Antitag Antibodies FP tags Antibodies
Anti-eGFP Tag Antibody
Specification
| Product Name | Rabbit polyclonal eGFP antibody |
| Clone Name | |
| IsoType | IgG |
| Concentration | 1mg/ml |
| Immunogen | Recombinant fragment contain a sequence corresponding to a region within amino acids 70 and 265 of GFP |
| Host Species | Rabbit |
| Formulation | 1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative |
| Reactivity | eGFP |
| Validated Application | WB, IF, IP |
| Suggested Dilution | |
| Predicted Size | 29.0KDa |
| Storage/Stability | Stable for 1 year at -20C from date of shipment. Aliquot to avoid repeated freezing and thawing. |
WB Image
Western blot analysis of GFP expression in transfected 293T cell line by GFP polyclonal antibody (12% SDS-PAGE, TA150032 diluted at 1:10000). A: Non-transfected lysate; B: GFP transfected lysate.
WB Image
Western blot analysis of 1 ug of whole cell lysate with anti-GFP antibody TA150032 diluted at 1:10000. A: Non-transfected 293T lysates; B: GFP transfected 293T lysates; C: RFP transfected 293T lysates.
IF Image
Immunofluorescence analysis of GFP-transfected HeLa. A:GFP is expressed in the tranfected cell. B: The cell expressing GFP can be detected using rabbit anti-GFP ab ( TA150032, 1:5000) followed by Alexa Fluor 594 (1:500) goat anti-rabbit IgG. C: Merged with DNA probe, the lower cell with no GFP expressed as a negtive control.
IP Image
GFP was immunoprecipitated from bacterial GFP-expressing lysate using 1 ug of anti-GFP antibody (TA150032, 1:15000).
|
