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Home Antibody All anti-ZAP70 antibodies

Anti-ZAP70 Antibody ER227(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307612
  • Rabbit monoclonal antibody against ZAP-70(clone ER227(2))
  • FREE positive control: HEK293T cell transient overexpression lysate (LC421504) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
Assay(1)
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Also for ZAP70 (NM_001079)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human ZAP-70 was used as an immunogen.
Clone NameER227(2) IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC: 1:250 - 1:500
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1
Alternative NameSRK; STCD; STD; TZK; ZAP-70
Database LinkNP_001070
FunctionZAP-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell antigen receptor (TCR) activation (1). Its involvement in pre-TCR and TCR signaling affects the adaptive immune response and thymocyte development (2). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with the recruitment of ZAP-70, a critical event in T cell activation (3). Following TCR engagement, ZAP-70 is phosphorylated by several tyrosine residues in the activation loop of the catalytic domain (2). Phosphorylation of Tyr315 and Tyr319 is required for full activation and increased signal propagation of downstream target molecules by ZAP-70 (4).
Related Pathway

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WB Image
Western blot - ZAP70 antibody [ER227(2)]; All lanes : Anti-ZAP70 antibody [ER227(2)] at 1/10000 dilution.Lane 1 : Human T78 cell lysate.Lane 2 : Jurkat cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 70 kDa.
Assay Image
Other-Anti-ZAP70 antibody [ER227(2)](TA307612); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ZAP70 antibody [ER227(2)]; TA307612 at 1/250 dilution staining ZAP70 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

 

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