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Anti-ZAP70 Antibody ER227(2)
Also for ZAP70 (NM_001079)
|A synthetic peptide corresponding to residues in human ZAP-70 was used as an immunogen.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IHC
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt.
|Homo sapiens zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1|
|SRK; STCD; STD; TZK; ZAP-70|
Entrez Gene 7535 Human
|ZAP-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell antigen receptor (TCR) activation (1). Its involvement in pre-TCR and TCR signaling affects the adaptive immune response and thymocyte development (2). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with the recruitment of ZAP-70, a critical event in T cell activation (3). Following TCR engagement, ZAP-70 is phosphorylated by several tyrosine residues in the activation loop of the catalytic domain (2). Phosphorylation of Tyr315 and Tyr319 is required for full activation and increased signal propagation of downstream target molecules by ZAP-70 (4).|
|Protein KinaseDruggable Genome Natural killer cell mediated cytotoxicityT cell receptor signaling pathwayPrimary immunodeficiency|
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Western blot - ZAP70 antibody [ER227(2)]; All lanes : Anti-ZAP70 antibody [ER227(2)] at 1/10000 dilution.Lane 1 : Human T78 cell lysate.Lane 2 : Jurkat cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 70 kDa.
Other-Anti-ZAP70 antibody [ER227(2)](TA307612); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ZAP70 antibody [ER227(2)]; TA307612 at 1/250 dilution staining ZAP70 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.