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Home Antibody All anti-ZAP70 antibodies

Anti-ZAP70 PHOSPHO Antibody EPR1073


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SKU Description Amount Price Availability*  
TA303740 Rabbit monoclonal antibody against ZAP70 Phospho (pY292) (EPR1073 ) (phospho-specific) 100ul $325 In Stock
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OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding Tyrosine 292 of human ZAP70 was used as an immunogen. This antibody only detects ZAP70 phosphorylated on Tyrosine 292.
Clone NameEPR1073 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:5,000 -10,000; ICC: 1:250 -500; FC: 1:20
Predicted MW Explanation 70 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens zeta chain of T cell receptor associated protein kinase 70kDa (ZAP70), transcript variant 1
Alternative NameSRK; STCD; STD; TZK; ZAP-70
Database LinkNP_001070
Entrez Gene 7535 Human
FunctionThis gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq].
Related PathwayProtein KinaseDruggable Genome Natural killer cell mediated cytotoxicityT cell receptor signaling pathwayPrimary immunodeficiency

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ZAP70 (phospho Y292) antibody [EPR1073]; All lanes : Anti-ZAP70 (phospho Y292) antibody [EPR1073] at 1/10000 dilution.Lane 1 : Jurkat cell lysate (untreated).Lane 2 : Jurkat cell lysate, treated with pervanadate.Lysates/proteins at 10 ug per lane.Secondary.goat anit-rabbit HRP labeled IgG at 1/2000 dilution.Predicted band size : 70 kDa.Observed band size : 70 kDa.

FC Image
Flow Cytometry - ZAP70 (phospho Y292) antibody [EPR1073]; Flow cytometric analysis of permeabilized Jurkat cells, untreated (green), pervanadate treated (red) stained with TA303740, at 1/20 dilution (blue) and pervanadate-treated Jurkat cells using the same antibody preincubated with phospho- ZAP70 (Tyr 292) peptide (blue) or non-phospho- ZAP70 (Tyr 292)).


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