The antiserum was produced against synthesized non-phosphopeptide derived from human 14-3-3 ? around the phosphorylation site of serine 58 (R-S-SP-W-R).
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clone Name
Isotype
IgG
Species Reactivity
Human, Mouse, Rat
Concentration
1mg/ml
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
Guaranteed Application *
WB, IHC, IF
Suggested Dilutions
WB: 1:500~1000, IHC: 1:50~100, IP: Various DilutionIF: 1:100~1:500, ELISA: 1:40000
Background
14-3-3 . (Ab-58) antibody detects endogenous levels of total 14-3-3 . protein.
Related Pathway
Cell cycle
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Western blot analysis of extracts from 293 cells treated with Forskolin (40nM, 30min) using 14-3-3 ? (Ab-58) antibody (#B0001, Line1 and 2).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 ? (Ab-58) antibody (#B0001).The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using 14-3-3 ? (Ab-58) antibody (#B0001).The picture on the right is treated with the synthesized peptide.
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