The antiserum was produced against synthesized non-phosphopeptide derived from human 14-3-3 ß/? around the phosphorylation site of serine 184/186 (L-N-SP-P-E).
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clone Name
Isotype
IgG
Species Reactivity
Human, Mouse, Rat
Concentration
1mg/ml
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
WB: 1:500~1:3000, ELISA: 1:20000
Background
14-3-3 ./. (Ab-184/186) antibody detects endogenous levels of total 14-3-3 ./. protein.
Related Pathway
Cell cycle
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Western blot analysis of extracts from 3T3 cells, using 14-3-3 ß/? (Ab-184/186) antibody.The lane on the right is treated with the synthesized peptide.
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