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Home Antibody All anti-XRCC6 antibodies

Anti-XRCC6 Antibody EPR4027

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307538
  • Rabbit monoclonal antibody against Ku70(clone EPR4027)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400567) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for XRCC6 (NM_001469)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Ku70 was used as an immunogen.
Clone NameEPR4027 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsFC: 1:10 - 100, ICC: 1:100 - 250, IHC: 1:100 - 250, IP: 1:10 - 100, WB: 1:1,000-10,000,
Predicted MW Explanation 70 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens X-ray repair complementing defective repair in Chinese hamster cells 6 (XRCC6), transcript variant 1
Alternative NameCTC75; CTCBF; G22P1; KU70; ML8; TLAA
Database LinkNP_001460
Entrez Gene 2547 Human
FunctionATP-dependent DNA helicase 2 subunit 1 (Ku70) is a single stranded DNA-dependent helicase originally recognized by the sera of patients with autoimmune diseases (1, 2). It plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination (3). Ku70 forms a heterodimer with Ku80 and contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining (NHEJ) pathway (4). The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex, DNA-PK (1). Together, the Ku70/Ku80 heterodimer and DNA-PKcs are required for V(D)J recombination and DNA double-strand break repair and may also play a role in transcription regulation (5). In addition, the dimer associates with NARG1 to bind to the osteocalcin promoter and activate osteocalcin expression (1).
Related PathwayTranscription FactorsDruggable Genome Non-homologous end-joining

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Ku70 antibody [EPR4027]; All lanes : Anti-Ku70 antibody [EPR4027] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : A549 cell lysate.Lane 4 : A431 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 70 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ku70 antibody [EPR4027]; Paraffin embedded Human testis tissue (A) or Human tonsil tissue (B) were labelled with TA307538 at 1/100 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - Ku70 antibody [EPR4027]; Immunofluorescence staining of HeLa cells using TA307538 at 1/100 dilution.
FC Image
Flow Cytometry-Anti-Ku70 antibody [EPR4027](TA307538); Overlay histogram showing HeLa cells stained with TA307538 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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