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Anti-XRCC6 Antibody EPR4027
Also for XRCC6 (NM_001469)
|A synthetic peptide corresponding to residues in human Ku70 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||FC: 1:10 - 100, ICC: 1:100 - 250, IHC: 1:100 - 250, IP: 1:10 - 100, WB: 1:1,000-10,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens X-ray repair complementing defective repair in Chinese hamster cells 6 (XRCC6)|
|CTC75; CTCBF; G22P1; KU70; ML8; TLAA|
|ATP-dependent DNA helicase 2 subunit 1 (Ku70) is a single stranded DNA-dependent helicase originally recognized by the sera of patients with autoimmune diseases (1, 2). It plays a key role in multiple nuclear processes such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination (3). Ku70 forms a heterodimer with Ku80 and contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining (NHEJ) pathway (4). The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex, DNA-PK (1). Together, the Ku70/Ku80 heterodimer and DNA-PKcs are required for V(D)J recombination and DNA double-strand break repair and may also play a role in transcription regulation (5). In addition, the dimer associates with NARG1 to bind to the osteocalcin promoter and activate osteocalcin expression (1).|
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Western blot - Ku70 antibody [EPR4027]; All lanes : Anti-Ku70 antibody [EPR4027] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : A549 cell lysate.Lane 4 : A431 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 70 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ku70 antibody [EPR4027]; Paraffin embedded Human testis tissue (A) or Human tonsil tissue (B) were labelled with ab92450 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Ku70 antibody [EPR4027]; Immunofluorescence staining of HeLa cells using ab92450 at 1/100 dilution.
Flow Cytometry-Anti-Ku70 antibody [EPR4027](ab92450); Overlay histogram showing HeLa cells stained with ab92450 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.