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Anti-XRCC5 Antibody EPR3468

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SKU Description Amount Price Availability*  
TA307065
  • Rabbit monoclonal antibody against Ku80 (clone EPR3468 )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC412062) , 20ug
100ul $325 In Stock
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Ku80 was used as an immunogen.
Clone NameEPR3468 IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:50; IHC-P: 1:250 - 1:500; ICC/IF: 1:500 - 1:1000
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) (XRCC5)
Alternative NameKARP-1; KARP1; KU80; Ku86; KUB2; NFIV
Database LinkNP_066964
FunctionThe Ku antigen is a DNA-associated nuclear protein originally recognized by the sera of patients with autoimmune diseases (1). It plays a key role in multiple nuclear processes, such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination (2). The Ku protein is composed of 70 and 82 kDa subunits (Ku70 and Ku80, respectively) and contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway (3). When bound to DNA, the Ku 70/80 heterodimer enhances the kinase activity of the catalytic subunit of the DNA-dependent protein kinase, DNA-PKcs (4).
Related Pathway

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WB Image
Western blot - Ku80 antibody [EPR3468]; All lanes : Anti-Ku80 antibody [EPR3468] at 1/50000 dilution.Lane 1 : A549 cell lysate .Lane 2 : HeLa cell lysate.Lane 3 : HepG2 cell lysate.Lane 4 : MCF7 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 83 kDa.Observed band size : 83 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ku80 antibody [EPR3468]; Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using 1/100 TA307065.
IF Image
Immunocytochemistry/ Immunofluorescence - Ku80 antibody [EPR3468]; Immunofluorescence analysis of HeLa cells with 1/500 TA307065.

 

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