Home Antibody All anti-XAF1 antibodies
Also for XAF1 (NM_017523)
|XAF-1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human XAF-1.|
||WB: 0.5 - 2 ug/ml
|PBS containing 0.02% sodium azide.|
|Affinity chromatography purified via peptide column
|Homo sapiens XIAP associated factor 1 (XAF1), transcript variant 1|
|BIRC4BP; HSXIAPAF1; XIAPAF1|
Entrez Gene 54739 Human
Entrez Gene 327959 Mouse
|XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus (1,2). Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death (1). XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines (3). In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines (2,3). Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs (4).|
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Western blot analysis of XAF-1 in human spleen lysate with XAF-1 antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) ug/ml, respectively.
Immunohistochemical staining of human spleen tissue using XAF-1 antibody at 2 ug/ml.