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Anti-WT1 Antibody CAN-R9(IHC)-56-2
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|A recombinant protein corresponding to the N-terminus of human WT was used as an immunogen.|
||WB 1:1000-5,000; IHC 1:100-250
|Store at -20 C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA. Stable for 12 months from date of receipt.|
|Homo sapiens Wilms tumor 1 (WT1), transcript variant A|
|AWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT33|
|Wilms Tumor (WT) is a 55 kDa protein thought to play a major role in various cancers and developmental disorders, commonly kidney tumors and glomerular diseases. WT is a zinc finger transcription factor that is mutated in Wilms tumors and highly expressed in a wide variety of other malignancies (1). WT1 consists of two major isoforms; WT1 (-KTS), a transcription factor, and WT1 (+KTS) (2). Mutations in the zinc finger regions of WT are detected in nearly all patients of Denys-Drash syndrome and in some patients with isolated diffuse mesangial sclerosis. Also, mutations leading to the loss of the transcription factor isoform have been observed in all patients with Frasier syndrome.|
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Western blot analysis on Ramos cell lysate using anti-WT RabMab
Immunohistochemical analysis of paraffin-embedded human Fetal kidney tissue using anti-WT RabMAb
Immunohistochemical analysis of paraffin-embedded human Wilms Tumor tissue using anti-WT RabMAb