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Anti-WT1 Antibody CAN-R9(IHC)-56-2


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  • Rabbit Monoclonal Antibody for Wilm's Tumor
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424994) , 20ug Explanation
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OriGene Data

ImmunogenA recombinant protein corresponding to the N-terminus of human WT was used as an immunogen.
Clone NameCAN-R9(IHC)-56-2 IsotypeIgG
Species ReactivityMouse, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsIHC-Fr: 1:250; FC: 1:100; WB: 1:1000 - 1:5000; IHC-P: 1:100 - 1:250; ICC/IF: Use at an assay dependent concentration
Predicted MW Explanation 55 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens Wilms tumor 1 (WT1), transcript variant A
Alternative NameAWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT33
Database LinkNP_000369
Entrez Gene 7490 Human
Entrez Gene 22431 Mouse
FunctionWilms Tumor (WT) is a 55 kDa protein thought to play a major role in various cancers and developmental disorders, commonly kidney tumors and glomerular diseases. WT is a zinc finger transcription factor that is mutated in Wilms tumors and highly expressed in a wide variety of other malignancies (1). WT1 consists of two major isoforms; WT1 (-KTS), a transcription factor, and WT1 (+KTS) (2). Mutations in the zinc finger regions of WT are detected in nearly all patients of Denys-Drash syndrome and in some patients with isolated diffuse mesangial sclerosis. Also, mutations leading to the loss of the transcription factor isoform have been observed in all patients with Frasier syndrome.
Related PathwayTranscription FactorsDruggable Genome

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WB Image
Western blot - Wilms Tumor Protein antibody; Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] at 1/1000 dilution + Cell lysates prepared from Ramos cells.Predicted band size : 55 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wilms Tumor Protein antibody; TA305610 at 1/250 dilution staining Wilms Tumor Protein in human fetal tissue sections by Immunohistochemistry (Formalin fixed Paraffin-embedded tissue sections).
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wilms Tumor Protein antibody; TA305610 at 1/250 dilution staining Wilms Tumor Protein in human Wilms tumor tissue section by IHC-P.
FC Image
Flow Cytometry-Anti-Wilms Tumor Protein antibody(TA305610); Overlay histogram showing K562 cells stained with TA305610 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.


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