OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-WT1 antibodies

Anti-WT1 Antibody CAN-R9(IHC)-56-2

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA305610 Rabbit Monoclonal Antibody for Wilm's Tumor 100ul $325 In Stock
LC424994 WT1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

spacer
WB(1)
IHC(2)
FC(1)

OriGene Data

ImmunogenA recombinant protein corresponding to the N-terminus of human WT was used as an immunogen.
Clone NameCAN-R9(IHC)-56-2 IsotypeIgG
Species ReactivityMouse, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsIHC-Fr: 1:250; FC: 1:100; WB: 1:1000 - 1:5000; IHC-P: 1:100 - 1:250; ICC/IF: Use at an assay dependent concentration
Predicted MW Explanation 55 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens Wilms tumor 1 (WT1), transcript variant A
Alternative NameAWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT33
Database LinkNP_000369
Entrez Gene 7490 Human
Entrez Gene 22431 Mouse
FunctionWilms Tumor (WT) is a 55 kDa protein thought to play a major role in various cancers and developmental disorders, commonly kidney tumors and glomerular diseases. WT is a zinc finger transcription factor that is mutated in Wilms tumors and highly expressed in a wide variety of other malignancies (1). WT1 consists of two major isoforms; WT1 (-KTS), a transcription factor, and WT1 (+KTS) (2). Mutations in the zinc finger regions of WT are detected in nearly all patients of Denys-Drash syndrome and in some patients with isolated diffuse mesangial sclerosis. Also, mutations leading to the loss of the transcription factor isoform have been observed in all patients with Frasier syndrome.
Related PathwayTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Wilms Tumor Protein antibody; Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] at 1/1000 dilution + Cell lysates prepared from Ramos cells.Predicted band size : 55 kDa.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wilms Tumor Protein antibody; TA305610 at 1/250 dilution staining Wilms Tumor Protein in human fetal tissue sections by Immunohistochemistry (Formalin fixed Paraffin-embedded tissue sections).
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wilms Tumor Protein antibody; TA305610 at 1/250 dilution staining Wilms Tumor Protein in human Wilms tumor tissue section by IHC-P.
spacer
Flow Cytometry-Anti-Wilms Tumor Protein antibody(TA305610); Overlay histogram showing K562 cells stained with TA305610 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title