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Anti-WT1 Antibody CAN-R9(IHC)-56-2

* Buy one primary antibody, get 50% off a GAPDH loading control antibody.

WB(1)

IHC(2)

OriGene Data

Immunogen	A recombinant protein corresponding to the N-terminus of human WT was used as an immunogen.
Clone Name	CAN-R9(IHC)-56-2	Isotype	IgG
Species Reactivity	Human	Concentration
Guaranteed Application *	WB, IHC	Suggested Dilutions	WB 1:1000-5,000; IHC 1:100-250
Buffer	Store at -20 C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA. Stable for 12 months from date of receipt.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens Wilms tumor 1 (WT1), transcript variant A
Alternative Name	AWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT33
Database Link	NP_000369
Function	Wilms Tumor (WT) is a 55 kDa protein thought to play a major role in various cancers and developmental disorders, commonly kidney tumors and glomerular diseases. WT is a zinc finger transcription factor that is mutated in Wilms tumors and highly expressed in a wide variety of other malignancies (1). WT1 consists of two major isoforms; WT1 (-KTS), a transcription factor, and WT1 (+KTS) (2). Mutations in the zinc finger regions of WT are detected in nearly all patients of Denys-Drash syndrome and in some patients with isolated diffuse mesangial sclerosis. Also, mutations leading to the loss of the transcription factor isoform have been observed in all patients with Frasier syndrome.
Related Pathway

Related Lysate from OriGene • Transient overexpression lysate of Wilms tumor 1 (WT1), transcript variant A • Transient overexpression lysate of Wilms tumor 1 (WT1), transcript variant A Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • WT1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • WT1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Wt1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • WT1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Wt1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens Wilms tumor 1 (WT1), transcript variant A as transfection-ready DNA • Lenti ORF clone of Human Wilms tumor 1 (WT1), transcript variant A , Myc-DDK-tagged • Lenti ORF clone of Human Wilms tumor 1 (WT1), transcript variant A, mGFP tagged • GFP-tagged ORF clone of Homo sapiens Wilms tumor 1 (WT1), transcript variant A as transfection-ready DNA Related cDNA Clones • Homo sapiens Wilms tumor 1 (WT1), transcript variant A as transfection-ready DNA Functional Proteins • WT-1 (-KTS), The Wilms’ Tumor Suppressor and Transcription/Pre-mRNA Splicing Factor 5,000 units • WT-1 (-KTS), The Wilms’ Tumor Suppressor and Transcription/Pre-mRNA Splicing Factor 12,500 units

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

• HRP
• Biotin
• PE
• FITC
• DyLight® 488
• DyLight® 550
• DyLight® 594
• DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)