Comparison of the dose–response relationship of radiation-induced apoptosis in the hippocampal dentate gyrus and intestinal crypt of adult mice
Radiat Prot Dosimetry, Mar 2012; 148: 492 - 497.
[LGR5
]
Tracking protein aggregation and mislocalization in cells with flow cytometry
Nature Methods doi:10.1038/nmeth.1930
[HSPA1A]
Histone deacetylase inhibitors prevent the degradation and restore the activity of glucocerebrosidase in Gaucher disease
PNAS, Dec 2011; 108: 21200 - 21205.
[anti-DDK antibody]
Homo sapiens Wiskott-Aldrich syndrome (eczema-thrombocytopenia) (WAS)
Synonyms:
IMD2; THC; THC1; WASP
Immunogen
Peptide with sequence C-SPADKKRSGKKKI, from the internal region of the protein sequence according to NP_000368.1.
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clone Name
Isotype
Goat IgG
Species Reactivity
Human
Concentration
0.5 mg/ml
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
ELISA: 1:128,000. WB: 0.03-0.1µg/ml.
Background
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq].
Related Pathway
Adherens junction
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TA303004 staining (0.03µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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