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Anti-WAPAL Antibody EPR3407(2)


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SKU Description Amount Price Availability*  
  • Rabbit Monoclonal antibody against WAPL (WAPAL)
100ul 325 3-7 Days
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human WAPL was used as an immunogen.
Clone NameEPR3407(2) IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:50000 - 1:200000; IP: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or IHC-P.

Reference Data

Target NameHomo sapiens wings apart-like homolog (Drosophila) (WAPAL)
Alternative NameFOE; KIAA0261; WAPL
Database LinkNP_055860
Entrez Gene 23063 Human
FunctionWAPL, also known as Wings apart-like protein homolog, is a cohesion-binding protein that regulates both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis (1, 2). Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair (1).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - WAPL antibody [EPR3407(2)]; All lanes : Anti-WAPL antibody [EPR3407(2)] at 1/50000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : Jurkat cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 133 kDa.
WB Image
Western blot - Anti-WAPL antibody [EPR3407(2)]; All lanes : Anti-WAPL antibody [EPR3407(2)] at 1/10000 dilution.Lane 1 : Human U2OS cells treated with siRNA control.Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL. .Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL.Lysates/proteins at 90 ug per lane.Secondary. Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 133 kDa.Observed band size : 150 kDa .Additional bands at : 120 kDa (possible cross reactivity),130 kDa (possible cross reactivity),145 kDa (possible cross reactivity).
IF Image
Immunocytochemistry/ Immunofluorescence - WAPL antibody [EPR3407(2)]; TA311107 at 1/100 dilution staining WAPL in HeLa cells by Immunofluorescence.


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