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Home Antibody All anti-VPS35 antibodies

Anti-VPS35 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302699
  • Goat Polyclonal Antibody against VPS35 / MEM3
  • FREE positive control: HEK293T cell transient overexpression lysate (LC402657) , 20ug
100ug $325 In Stock
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WB(1)
IHC(1)
IF(2)

OriGene Data

Immunogen Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.
Clone Name IsotypeGoat IgG
Species ReactivityHuman Concentration0.5mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsELISA: 1:32,000; WB: 0.01-0.1ug/ml
BufferSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Note In paraffin embedded Human Testis, shows positive staining in Leydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymis and of the rete testis. Data obtained with a previous batch.

Reference Data

Target NameHomo sapiens vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35)
Alternative NameMEM3; PARK17
Database LinkNP_060676
FunctionThis gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
Related Pathway

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WB Image
TA302699 staining (0.05µg/ml) of Human Brain Cerebellum lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
IHC Image
TA302699 staining of paraffin embedded Human Testis showing A) Epithelial cells of the epididymis and B) Some leydig cells. Microwaved antigen retrieval with A)Tris/EDTA buffer pH9 at 3µg/ml or B)Citrate buffer pH6 at 10ug/ml., HRP-staining.
IF Image
TA302699 staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.
IF Image
TA302699 staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.

 

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