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Anti-VIM PHOSPHO Antibody EP1071Y
|A synthetic acetylated peptide corresponding to residues surrounding Ser82 of Vimentin was used as an immunogen. The antibody only detects Vimentin phosphorylated at Serine 82.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens vimentin (VIM)|
|Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]. |
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Western blot - Vimentin (phospho S82) antibody [EP1071Y]; All lanes : Anti-Vimentin (phospho S82) antibody [EP1071Y] at 1/500000 dilution.Lane 1 : HeLa cell lysate AP treated.Lane 2 : HeLa cell lysate (AP untreated) + Calyculin A.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 54 kDa.Observed band size : 54 kDa.