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Home Antibody All anti-VCL antibodies

Anti-VCL Antibody EPR8185

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA311103
  • Rabbit Monoclonal antibody against Vinculin (VCL)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418728) , 20ug
100ul 325 3-7 Days
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WB(1)
IF(1)
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Also for VCL (NM_003373)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Vinculin was used as an immunogen.
Clone NameEPR8185 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IHC-P.

Reference Data

Target NameHomo sapiens vinculin (VCL), transcript variant 2
Alternative NameCMD1W; CMH15; HEL114; MV; MVCL
Database LinkNP_003364
FunctionVinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in Vinculin are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia (1).
Related Pathway
Adherens junction
Focal Adhesion
Hemostasis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Vinculin antibody [EPR8185]; All lanes : Anti-Vinculin antibody [EPR8185] at 1/10000 dilution.Lane 1 : PC3 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : U937 cell lysate.Lane 4 : K562 cell lysate.Lane 5 : HUVEC cell lysate.Lane 6 : Human fetal liver lysate.Lane 7 : Human fetal kidney lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 124 kDa.Observed band size : 124 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [EPR8185]; TA311103, at 1/100 dilution staining Vinculin in HUVEC cells by Immunofluorescence.

 

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