A phosphor synthetic peptide corresponding to residues surrounding Tyrosine 174 of human Vav was used as an immnogen. This antibody detects Vav phosphorylated at Tyrosine 174.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels (2). As a signaling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein (3). Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response (3).
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
Hemostasis
Signaling by GPCR
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Western blot analysis on Jurkat cell lysates using anti-Phospho-Vav (pY174), 1:500,000 dilution. Cells were either (A) untreated (B) treated with pervanadate.
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